Answer in Organic Chemistry for Cameron Hicks #121582
April 21st, 2023
Coelenterazine is used in a reaction with a Luciferase enzyme, to form Coelenteramide. A single carbon has been removed from Coelenterazine to from Coelenteramide. How would you go about converting Coelenteramide back into its original form for reuse?
The substrate of Renilla luciferase, coelenterazine, consists of a central aromatic imidazopyrazinone, which is derivatized with p-hydroxy-phenyl (R1), benzyl (R2), and p-hydroxy-benzyl (R3) moieties. Using molecular oxygen, RLUC catalyzes an oxidative decarboxylation in which the imidazole ring is opened and carbon dioxide is released